Preparation of Berbamine Loaded Chitosan-agarose Microspheres and in vitro Release Study
Hu, Zhang; Li, Sidong; Yang, Lei
http://dx.doi.org/10.1590/S0104-14282012005000073
Polímeros: Ciência e Tecnologia, vol.22, n5, p.422-426, 2012
Abstract
Berbamine loaded chitosan-agarose microspheres were prepared using a water-in-oil emulsion technique. Optimum preparing parameters were determined by orthogonal experiments as follows: ratio of berbamine to chitosan (w/w) is 1:10; percentage of emulsifier (span 80, v/v) is 6%; volume of glutaraldehyde is 2 mL; and reaction temperature is 70 °C. Under these optimal conditions, the encapsulation efficiency and loading capacity of microspheres are 84.57% and 8.44%, respectively. The swelling tests showed that the microspheres possessed higher swelling ratio at pH 7.4 than at pH 1.2. FTIR indicated that berbamine had been successfully loaded in the chitosan-agarose microspheres by physical entrapment. In vitro release studies showed that berbamine was released from microspheres in a significantly sustained fashion.
Keywords
Chitosan, agarose, microspheres, berbamine
References
1. Xu, R. Z.; Dong, Q. H.; Yu, Y.; Zhao, X.; Gan, X.; Wu, D.; Lu, Q.; Xu,
X. & Yu, X. F. - Leuk. Res., 30, p.17 (2006). PMid:16023722. http://
dx.doi.org/10.1016/j.leukres.2005.05.023
2. Wei, Y.; Xu, L.; Liang, Y.; Xu, X. & Zhao, X. - Acta Pharmacol. Sin., 30, p.451 (2009). PMid:19270722. http://dx.doi.org/10.1038/aps.2009.19
3. Foster, N. & Hirst, B. - Adv. Drug Deliver. Rev., 57, p.431 (2005). PMid:15560950. http://dx.doi.org/10.1016/j.addr.2004.09.009
4. Tamber, H.; Johansen, P.; Merkle, H. P. & Gander, B. - Adv. Drug Deliver. Rev., 57, p.357 (2005). PMid:15560946. http://dx.doi. org/10.1016/j.addr.2004.09.002
5. Rinaudo, M. - Prog. Polym. Sci., 31, p.603 (2006). http://dx.doi. org/10.1016/j.progpolymsci.2006.06.001
6. Ravi Kumar, M. N. V.; Muzzarelli, R. A. A.; Muzzarelli, C.; Sashiwa, H. & Domb, A. J. - Chem. Rev., 104, p.6017 (2004).
7. Qi, L. F.; Xu, Z. R.; Jiang, X.; Li, Y. & Wang, M. Q. - Bioorg. Med. Chem. Lett., 15, p.1397 (2005). PMid:15713395. http://dx.doi. org/10.1016/j.bmcl.2005.01.010
8. Qi, L. F. & Xu, Z. R. - Bioorg. Med. Chem. Lett., 16, p.4243 (2006). PMid:16759859. http://dx.doi.org/10.1016/j.bmcl.2006.05.078
9. Sinha, V. R.; Singal, A. K.; Wadhawan, S.; Kaushik, R.; Kumria, K.; Bansal, K. & Dhawan, S. - Int. J. Pharmaceut., 274, p.1 (2004). PMid:15072779. http://dx.doi.org/10.1016/j.ijpharm.2003.12.026
10. Deszczynski, M.; Kasapis, S. & Mitchelle, J. R. - Carbohyd. Polym., 53, p.85 (2003). http://dx.doi.org/10.1016/S0144-8617(02)00327-2
11. Lahooti, S. & Sefton, M. V. - Biomaterials, 21, p.987 (2000). http:// dx.doi.org/10.1016/S0142-9612(99)00251-3
12. Watase, M.; Nishinari, K.; Clark, A. H. & Ross-Murphy, S. B. - Macromolecules, 22, p.1196 (1989). http://dx.doi.org/10.1021/ ma00193a034
13. Xue, Z. X.; Yang, G. P.; Wang, G. C.; Niu, J. F. & Cao, X. Y. - J. Appl. Polym. Sci., 103, p.2759 (2007). http://dx.doi.org/10.1002/app.25335
14. Lim, S. H. & Hudson, S. M. - Carbohydr. Res., 339, p.313 (2004). PMid:14698889. http://dx.doi.org/10.1016/j.carres.2003.10.024
15. Çetinus, Ş. A.; Öztop, H. N. & Saraydin, D. - Enzyme Microb. Tech., 41, p.447 (2007). http://dx.doi.org/10.1016/j.enzmictec.2007.03.014
16. Garcia, R. B. & Vidal, R. R. L. - Polímeros, 10, p.155 (2000). http:// dx.doi.org/10.1590/S0104-14282000000300012
17. Liu, G. S.; Chen, B. Z.; Song, W. Z. & Xiao, P. G. - Acta Botanica Sin., 20, p. 9 (1978).
2. Wei, Y.; Xu, L.; Liang, Y.; Xu, X. & Zhao, X. - Acta Pharmacol. Sin., 30, p.451 (2009). PMid:19270722. http://dx.doi.org/10.1038/aps.2009.19
3. Foster, N. & Hirst, B. - Adv. Drug Deliver. Rev., 57, p.431 (2005). PMid:15560950. http://dx.doi.org/10.1016/j.addr.2004.09.009
4. Tamber, H.; Johansen, P.; Merkle, H. P. & Gander, B. - Adv. Drug Deliver. Rev., 57, p.357 (2005). PMid:15560946. http://dx.doi. org/10.1016/j.addr.2004.09.002
5. Rinaudo, M. - Prog. Polym. Sci., 31, p.603 (2006). http://dx.doi. org/10.1016/j.progpolymsci.2006.06.001
6. Ravi Kumar, M. N. V.; Muzzarelli, R. A. A.; Muzzarelli, C.; Sashiwa, H. & Domb, A. J. - Chem. Rev., 104, p.6017 (2004).
7. Qi, L. F.; Xu, Z. R.; Jiang, X.; Li, Y. & Wang, M. Q. - Bioorg. Med. Chem. Lett., 15, p.1397 (2005). PMid:15713395. http://dx.doi. org/10.1016/j.bmcl.2005.01.010
8. Qi, L. F. & Xu, Z. R. - Bioorg. Med. Chem. Lett., 16, p.4243 (2006). PMid:16759859. http://dx.doi.org/10.1016/j.bmcl.2006.05.078
9. Sinha, V. R.; Singal, A. K.; Wadhawan, S.; Kaushik, R.; Kumria, K.; Bansal, K. & Dhawan, S. - Int. J. Pharmaceut., 274, p.1 (2004). PMid:15072779. http://dx.doi.org/10.1016/j.ijpharm.2003.12.026
10. Deszczynski, M.; Kasapis, S. & Mitchelle, J. R. - Carbohyd. Polym., 53, p.85 (2003). http://dx.doi.org/10.1016/S0144-8617(02)00327-2
11. Lahooti, S. & Sefton, M. V. - Biomaterials, 21, p.987 (2000). http:// dx.doi.org/10.1016/S0142-9612(99)00251-3
12. Watase, M.; Nishinari, K.; Clark, A. H. & Ross-Murphy, S. B. - Macromolecules, 22, p.1196 (1989). http://dx.doi.org/10.1021/ ma00193a034
13. Xue, Z. X.; Yang, G. P.; Wang, G. C.; Niu, J. F. & Cao, X. Y. - J. Appl. Polym. Sci., 103, p.2759 (2007). http://dx.doi.org/10.1002/app.25335
14. Lim, S. H. & Hudson, S. M. - Carbohydr. Res., 339, p.313 (2004). PMid:14698889. http://dx.doi.org/10.1016/j.carres.2003.10.024
15. Çetinus, Ş. A.; Öztop, H. N. & Saraydin, D. - Enzyme Microb. Tech., 41, p.447 (2007). http://dx.doi.org/10.1016/j.enzmictec.2007.03.014
16. Garcia, R. B. & Vidal, R. R. L. - Polímeros, 10, p.155 (2000). http:// dx.doi.org/10.1590/S0104-14282000000300012
17. Liu, G. S.; Chen, B. Z.; Song, W. Z. & Xiao, P. G. - Acta Botanica Sin., 20, p. 9 (1978).
Facebook
Google+
Twitter
LinkedIn
Mendeley
StumbleUpon
CiteULike
Reddit
Email