Chitosan as Flocculant Agent for Clarification of Stevia Extract
Oliveira, Silvia P. D. de; Mahl, Cynthia R. A.; Simões, Márcia R.; Silva, Classius F. da
http://dx.doi.org/10.1590/S0104-14282012005000066
Polímeros: Ciência e Tecnologia, vol.22, n4, p.401-406, 2012
Abstract
Stevia is used as a sweetener due to its low calorific value and its taste, which is very similar to that of sucrose. After extraction from dried leaves, stevia extract is dark in colour, and therefore needs to be whitened to increase acceptance by consumers. In this study we tested chitosan, a cationic polyelectrolyte, as flocculant agent for the whitening of the Stevia extract. Positive charges of chitosan can interact electrostatically with a counter-ion, sodium tripolyphosphate (TPP), and then chitosan precipitates. A factorial design was used to study the whitening process, in which Glycosides Removal, Colour Removal, Turbidity Removal and Soluble Solids Removal were evaluated. The studied factors were Chitosan Mass and pH of the TPP solution. The results showed that chitosan is a good flocculant agent, being able to flocculate both the glycosides and the pigments that make the extract coloured.
Keywords
Stevia, sweeteners, chitosan, flocculation, clarification
References
1. Brandle, J. E.; Starratt, A. N. & Gijzen, M. - Can. J. Plant Sci., 78,
p.527 (1998). http://dx.doi.org/10.4141/cjps10086
2. Rajagopal, R. - “Stevia as an alternative sweetener – Promise and Challenges”, KnowGenix & Centre for Management Technology (2009). Disponível em:. Accesso em: 5 fev. 2011.
3. Crammer, B. & Ikan, R. - “Progress in the Chemistry and Properties of Rebaudiosides”, in: Developments in Sweeteners, v.3, T. H. Grenby (ed.), Elsevier Applied Science, London (1987).
4. Phillips, K. C. - “Stevia: Steps in Developing a New Sweetener”, in: Developments in Sweeteners, v.3, T. H. Grenby (ed.), Elsevier Applied Science, London (1987).
5. Takamura, K.; Kawaguchi, M. & Isono, C. - “Stevioside from Stevia rebaudiana”, Japanese Patent No 52047961 (in japanese) (1977).
6. Abou-Arab, A. E., Abou-Arab, A. A. & Abu-Salem, M. F. - African Journal of Food Science, 4(5), p.269 (2010).
7. Silva, F. V.; Bergamasco, R.; Andrade, C. M. G.; Pinheiro, N.; Machado, N. R. C. F.; Reis M. H.; Araújo, Á. A. & Rezende, S. - Int. J. Chem. React. Eng., 5, p.A40 (2007). http://dx.doi.org/10.2202/1542-6580.1443
8. Reis, M. H. M.; Silva, F. V.; Andrade, C. M. G.; Rezende, S. L.; Maciel, M. R. W. & Bergamasco, R. - J. Food Proc. Eng., 32, p.338 (2009).
9. Fernandes, L. M.; Pereira, N. C.; Mendes, E. S.; Lima, C. O. M. & Costa, S. C. - Acta Sci., 23, p.1369 (2001).
10. Bunhak, E. J.; Mendes, E. S.; Pereira, N. C. & Costa, S. C. - Acta Sci., 24, p.1643 (2002).
11. Mantovaneli, I. C. C.; Ferretti, E. C.; Simões, M. R. & Silva, C. F. - Braz. J. Chem. Eng., 21, p.449 (2004). http://dx.doi.org/10.1590/ S0104-66322004000300009
12. Mahl, C. R. A.; Oliveira, S. P. D.; Simões, M. R. & Silva, C. F. - Adsorption Sci. & Tech., 28, p.189 (2010).
13. Tharanathan, R. N. & Kittur, F. S. - Crit. Rev. Food Sci. & Nutr., 43, p.61 (2003). http://dx.doi.org/10.1080/10408690390826455
14. Synowiecki, J. & Al-Khatteb, N. A. A. - Crit. Rev. Food Sci. Nutr., 43, p.145 (2003). http://dx.doi.org/10.1080/10408690390826473
15. Guibal, E.; Vooren, V. M.; Dempsey, B. A. & Roussy, J. - Sep. Sci. Tech., 41, p.2487 (2006).
16. Zeng, D.; Wu, J. & Kennedy, J. F. - Carbohydr. Polym., 71, p.135 (2008). http://dx.doi.org/10.1016/j.carbpol.2007.07.039
17. Renault, F.; Sancey, B.; Charles, J.; Morin-Crini, N.; Badot, P-M; Winterton, P. & Crini, G. - Chem. Eng. J., 155, p.775 (2009). http:// dx.doi.org/10.1016/j.cej.2009.09.023
18. Godos, I.; Guzman, H. O.; Soto, R.; García-Encina, P. A.; Becares, E.; Muñoz, R. & Vargas, V. A. - Biores. Tech., 102, p.923 (2011). http:// dx.doi.org/10.1016/j.biortech.2010.09.036
19. Wang, J.-P.; Chen, Y.-Z.; Ge, X.-W & Yu H.-Q. - Coll. & Surf. A: Phys. Eng. Asp., 302, p.204 (2007).
20. Fuh, W. S. & Chiang, B. H. - J. Food Sci., 55, p.1454 (1990). http:// dx.doi.org/10.1111/j.1365-2621.1990.tb03956.x
21. Costa, S. C.; Nishiyama, P.; Kusumoto, I. T.; Vieira, L. G. E. & Alavarez, M. - Arq. Biol. Tecn., 34, p.425 (1991)
22. Haaland, P. D. - “Experimental design in biotechnology”, Marcel Dekker Inc, New York (1989).
23. Renault, F.; Sancey, B.; Badot, P.-M. & Crini, G. - Eur. Pol. J., 45, p.1337 (2009). http://dx.doi.org/10.1016/j.eurpolymj.2008.12.027
2. Rajagopal, R. - “Stevia as an alternative sweetener – Promise and Challenges”, KnowGenix & Centre for Management Technology (2009). Disponível em:
3. Crammer, B. & Ikan, R. - “Progress in the Chemistry and Properties of Rebaudiosides”, in: Developments in Sweeteners, v.3, T. H. Grenby (ed.), Elsevier Applied Science, London (1987).
4. Phillips, K. C. - “Stevia: Steps in Developing a New Sweetener”, in: Developments in Sweeteners, v.3, T. H. Grenby (ed.), Elsevier Applied Science, London (1987).
5. Takamura, K.; Kawaguchi, M. & Isono, C. - “Stevioside from Stevia rebaudiana”, Japanese Patent No 52047961 (in japanese) (1977).
6. Abou-Arab, A. E., Abou-Arab, A. A. & Abu-Salem, M. F. - African Journal of Food Science, 4(5), p.269 (2010).
7. Silva, F. V.; Bergamasco, R.; Andrade, C. M. G.; Pinheiro, N.; Machado, N. R. C. F.; Reis M. H.; Araújo, Á. A. & Rezende, S. - Int. J. Chem. React. Eng., 5, p.A40 (2007). http://dx.doi.org/10.2202/1542-6580.1443
8. Reis, M. H. M.; Silva, F. V.; Andrade, C. M. G.; Rezende, S. L.; Maciel, M. R. W. & Bergamasco, R. - J. Food Proc. Eng., 32, p.338 (2009).
9. Fernandes, L. M.; Pereira, N. C.; Mendes, E. S.; Lima, C. O. M. & Costa, S. C. - Acta Sci., 23, p.1369 (2001).
10. Bunhak, E. J.; Mendes, E. S.; Pereira, N. C. & Costa, S. C. - Acta Sci., 24, p.1643 (2002).
11. Mantovaneli, I. C. C.; Ferretti, E. C.; Simões, M. R. & Silva, C. F. - Braz. J. Chem. Eng., 21, p.449 (2004). http://dx.doi.org/10.1590/ S0104-66322004000300009
12. Mahl, C. R. A.; Oliveira, S. P. D.; Simões, M. R. & Silva, C. F. - Adsorption Sci. & Tech., 28, p.189 (2010).
13. Tharanathan, R. N. & Kittur, F. S. - Crit. Rev. Food Sci. & Nutr., 43, p.61 (2003). http://dx.doi.org/10.1080/10408690390826455
14. Synowiecki, J. & Al-Khatteb, N. A. A. - Crit. Rev. Food Sci. Nutr., 43, p.145 (2003). http://dx.doi.org/10.1080/10408690390826473
15. Guibal, E.; Vooren, V. M.; Dempsey, B. A. & Roussy, J. - Sep. Sci. Tech., 41, p.2487 (2006).
16. Zeng, D.; Wu, J. & Kennedy, J. F. - Carbohydr. Polym., 71, p.135 (2008). http://dx.doi.org/10.1016/j.carbpol.2007.07.039
17. Renault, F.; Sancey, B.; Charles, J.; Morin-Crini, N.; Badot, P-M; Winterton, P. & Crini, G. - Chem. Eng. J., 155, p.775 (2009). http:// dx.doi.org/10.1016/j.cej.2009.09.023
18. Godos, I.; Guzman, H. O.; Soto, R.; García-Encina, P. A.; Becares, E.; Muñoz, R. & Vargas, V. A. - Biores. Tech., 102, p.923 (2011). http:// dx.doi.org/10.1016/j.biortech.2010.09.036
19. Wang, J.-P.; Chen, Y.-Z.; Ge, X.-W & Yu H.-Q. - Coll. & Surf. A: Phys. Eng. Asp., 302, p.204 (2007).
20. Fuh, W. S. & Chiang, B. H. - J. Food Sci., 55, p.1454 (1990). http:// dx.doi.org/10.1111/j.1365-2621.1990.tb03956.x
21. Costa, S. C.; Nishiyama, P.; Kusumoto, I. T.; Vieira, L. G. E. & Alavarez, M. - Arq. Biol. Tecn., 34, p.425 (1991)
22. Haaland, P. D. - “Experimental design in biotechnology”, Marcel Dekker Inc, New York (1989).
23. Renault, F.; Sancey, B.; Badot, P.-M. & Crini, G. - Eur. Pol. J., 45, p.1337 (2009). http://dx.doi.org/10.1016/j.eurpolymj.2008.12.027