Proliferation of Human Mesenchymal Stem Cells Derived from Adipose Tissue on Polyurethanes with Tunable Biodegradability
Pereira, Iaci M.; Gomide, Viviane; Oréfice, Rodrigo L.; Leite, Maria de F.; Zonari, Alessandra A. C.; Goes, Alfredo de M.
http://dx.doi.org/10.1590/S0104-14282010005000040
Polímeros: Ciência e Tecnologia, vol.20, n4, p.280-286, 2010
Abstract
Polyurethanes (PUs) have been considered good candidates to be used in biomedical temporary devices that require mechanical properties comparable to soft tissues. However, toxicity of some PUs is still a concern, since these polyurethanes can contain potential toxic components and residual organic solvents derived from their synthesis. In this work, in vitro tests to measure viability and proliferation of human mesenchymal stem cells (hMSCs) in contact with PUs with tunable biodegradability were performed by employing MTT, alkaline phosphatase and collagen secretion assays. PUs were produced in an aqueous environment by employing isophorone diisocyanate/hydrazine (hard segment) and poly(caprolactone diol)/2,2-bis (hydroxymethyl) propionic acid (soft segment) as the main reagents. Three series of PUs having different soft segment contents were synthesized. These PUs had their chemical structure, morphology and hydrolytic degradation investigated. The rate of hydrolysis of the obtained PUs was tailored by modifying the soft segment content of the polymers. In vitro results showed that PUs can provide a satisfactory environment for the adhesion and proliferation of hMSCs.
Keywords
Polyurethane, articular cartilage, biodegradation, in-vitro degradation, mesenchymal stem cells.
References
1. Redman, S. N.; Oldfield, S. & Archer, C. W. – Eur. Cell. Mater., p.2 (2005).
2. Buckwalter, J. A. – J. Orthop. & Sport. Phy. Ther., 28, p.192 (2008).
3. Ratner, B. D.; Hoffmann, A. S.; Schoen, F. J.; Lemons, J. E. - “Biomaterials science: an introduction to biomaterials in medicine”, Elsevier (2004).
4. Nöth, U.; Tuli, R.; Osyczka, A. M.; Danielson, K. & Tuan, R. S. - Tissue Eng., 8, p.131 (2002).
5. Martino, A. D.; Sittinger, M. & Risbud, M. V. - Biomaterials, 26, p.5983 (2005).
6. Szycher, M. “Introduction. Szycher’s Handbook of Polyurethanes”, CRC Press, London (1999).
7. Yang, J. H.; Chun, B. C.; Chung, Y. C. & Cho, J. H. – Polymer, 44, p.3251 (2003).
8. Hong, J. H.; Jeon, H. J.; Yoo, J. H.; Yu, W. R. & Youk, J. H. – Polym. Degrad. Stab., 92, p.1186 (2007).
9. Feng, Y. K. & Li, C. Y. – Polym. Degrad. Stab., 91, p.1711 (2006).
10. Minnen, B. V.; Leeuwen, M. V.; Stegenga, B.; Zuidema, J.; Hissink, C. E.; Kooten, T. G. & Bos, R. R. M. – Mater. Sci. Mater. Med., 16, p.221 (2005).
11. Zuk, P. A.; Zhu, M.; Ashjian, P.; Ugarte, D. A.; Huang, J. I.; Mizuno, H.; Alfonso, Z. C.; Fraser, J. K.; Benhaim, P. & Hedrick, M. H. – Mol. Cell. Biol., 13, p.4279 (2002).
12. Awada, H. A.; Wickham, M. Q.; Leddy, H. A.; Gimble, J. M. & Guilak, F. - Biomaterials, 25, p.3211 (2004).
13. Amaral, M.; Costa, M. A.; Lopes, M. A.; Silva, R. F.; Santos, J. D. & Fernandes, M. H. – Biomaterials, 23, p.4897 (2002).
14. Reinert, H. T. & Jundt, J. – Histochem. Cell. Biol., 112, p.271 (1999).
15. Ayres, E.; Oréfice, R. L. & Yoshida, M. I. – Eur. Polym. J., 43, p.3510 (2007).
16. Chattopadhyay, D. K.; Sreedhar, B. & Raju, K. V. S. N. – Polymer, 47, p.3814 (2006).
17. Kim, B. K.; Lee, S. Y. & Xu, M. – Polyurethanes, 37, p.5781 (1996).
18. Marcos-Fernández, A.; Abraham, G. A.; Valentín, J. L. & San Román, J. – Polymer, 47, p.785 (2006).
19. Coates, J. P. – “A Practical Approach to the Interpretation of Infrared Spectra”, in: Encyclopedia of Analytical Chemistry, p.10815, Meyers, R. A. Ed., Wiley, Chichester (2000).
20. Feng, Y. K. & Li, C. Y. – Polym. Degrad. Stab., 91, p.1711 (2006).
21. Dillon, J. – “Infrared Spectroscopy Atlas of Polyurethanes (Including Model Compounds)”, Technomic Lancaster, USA (1989).
22. Chew, M. Y. L.; Goh, S. H.; Kang, L. H.; Kang, L. H. & Tan, N. – Build. Environ., 34, p.49 (1999).
23. Pompe, G.; Pohlers, A.; Pötschke, P. & Pionteck, J. – Polymer, 39, p.5147 (1998).
24. Mosmann, T. J. - Immunol. Methods, 65, p.55 (1983).
2. Buckwalter, J. A. – J. Orthop. & Sport. Phy. Ther., 28, p.192 (2008).
3. Ratner, B. D.; Hoffmann, A. S.; Schoen, F. J.; Lemons, J. E. - “Biomaterials science: an introduction to biomaterials in medicine”, Elsevier (2004).
4. Nöth, U.; Tuli, R.; Osyczka, A. M.; Danielson, K. & Tuan, R. S. - Tissue Eng., 8, p.131 (2002).
5. Martino, A. D.; Sittinger, M. & Risbud, M. V. - Biomaterials, 26, p.5983 (2005).
6. Szycher, M. “Introduction. Szycher’s Handbook of Polyurethanes”, CRC Press, London (1999).
7. Yang, J. H.; Chun, B. C.; Chung, Y. C. & Cho, J. H. – Polymer, 44, p.3251 (2003).
8. Hong, J. H.; Jeon, H. J.; Yoo, J. H.; Yu, W. R. & Youk, J. H. – Polym. Degrad. Stab., 92, p.1186 (2007).
9. Feng, Y. K. & Li, C. Y. – Polym. Degrad. Stab., 91, p.1711 (2006).
10. Minnen, B. V.; Leeuwen, M. V.; Stegenga, B.; Zuidema, J.; Hissink, C. E.; Kooten, T. G. & Bos, R. R. M. – Mater. Sci. Mater. Med., 16, p.221 (2005).
11. Zuk, P. A.; Zhu, M.; Ashjian, P.; Ugarte, D. A.; Huang, J. I.; Mizuno, H.; Alfonso, Z. C.; Fraser, J. K.; Benhaim, P. & Hedrick, M. H. – Mol. Cell. Biol., 13, p.4279 (2002).
12. Awada, H. A.; Wickham, M. Q.; Leddy, H. A.; Gimble, J. M. & Guilak, F. - Biomaterials, 25, p.3211 (2004).
13. Amaral, M.; Costa, M. A.; Lopes, M. A.; Silva, R. F.; Santos, J. D. & Fernandes, M. H. – Biomaterials, 23, p.4897 (2002).
14. Reinert, H. T. & Jundt, J. – Histochem. Cell. Biol., 112, p.271 (1999).
15. Ayres, E.; Oréfice, R. L. & Yoshida, M. I. – Eur. Polym. J., 43, p.3510 (2007).
16. Chattopadhyay, D. K.; Sreedhar, B. & Raju, K. V. S. N. – Polymer, 47, p.3814 (2006).
17. Kim, B. K.; Lee, S. Y. & Xu, M. – Polyurethanes, 37, p.5781 (1996).
18. Marcos-Fernández, A.; Abraham, G. A.; Valentín, J. L. & San Román, J. – Polymer, 47, p.785 (2006).
19. Coates, J. P. – “A Practical Approach to the Interpretation of Infrared Spectra”, in: Encyclopedia of Analytical Chemistry, p.10815, Meyers, R. A. Ed., Wiley, Chichester (2000).
20. Feng, Y. K. & Li, C. Y. – Polym. Degrad. Stab., 91, p.1711 (2006).
21. Dillon, J. – “Infrared Spectroscopy Atlas of Polyurethanes (Including Model Compounds)”, Technomic Lancaster, USA (1989).
22. Chew, M. Y. L.; Goh, S. H.; Kang, L. H.; Kang, L. H. & Tan, N. – Build. Environ., 34, p.49 (1999).
23. Pompe, G.; Pohlers, A.; Pötschke, P. & Pionteck, J. – Polymer, 39, p.5147 (1998).
24. Mosmann, T. J. - Immunol. Methods, 65, p.55 (1983).
Facebook
Google+
Twitter
LinkedIn
Mendeley
StumbleUpon
CiteULike
Reddit
Email