Blends of Agar/Galactomannan for Marubakaido Apple Rootstock Shoot Proliferation
Sierakowski, Maria Rita; Anjos, A.; Quoirin, M.; Lucyszyn, N.
http://dx.doi.org/10.1590/S0104-14282005000200017
Polímeros: Ciência e Tecnologia, vol.15, n2, p.146-150, 2005
Abstract
Galactomannans (GMs) extracted from seeds of a native Brazilian specie designated Cassia fastuosa (cassia) and from Cyamopsis tetragonolobus (guar gum – a commercial GM) were mixed with agar, in the proportion of 3/3 g.L-1 (w/w), and used as a gelling agent in Marubakaido apple rootstock (Malus prunifolia Borkh) micropropagation. The rheological measurements of the gels showed a good interaction between both hidrocolloids. The micropropagation results on these media were compared with behaviour of standard medium containing agar only (6 g.L-1). Results from in vitro experiments indicated enhanced proliferation of apple shoots and also the occurrence of hyperhydric shoots was lower in the modified gel-medium.
Keywords
Galactomannan, micropropagation, apple tree, gelled medium
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