Recuperação e reutilização do carbonato de propileno empregado no processo de extração de poli(3-hidroxibutirato)
Recovery and reuse of propylene carbonate used in the process of poly(3-hydroxybutyrate) extraction
Quines, Luci Kelin de Menezes; Schmidt, Melodi; Zanfonato, Kellen; Martinhago, Francielli Manenti Schmidell, Willibaldo; Aragão, Gláucia Maria Falcão de
Resumo
A extração de poli(3-hidroxibutirato) (P(3HB)) com a utilização de solventes tóxicos e voláteis é contraditória à ideia do emprego destes biopolímeros para minimizar as agressões ambientais causadas pelos polímeros petroquímicos. O carbonato de propileno (CP) possui baixa toxicidade e alto ponto de ebulição, o que garante baixo risco de perdas por evaporação e alto rendimento de recuperação, podendo assim ser reutilizado. O objetivo deste trabalho foi verificar o efeito da reutilização do CP, recuperado do processo de extração de P(3HB) produzido por Cupriavidus necator, no rendimento e pureza do biopolímero e avaliar as perdas de solvente durante as etapas do processo de extração. Verificou-se a possibilidade de recuperar 80% do CP ao longo do processo. O solvente recuperado foi reutilizado onze vezes sem perder sua eficiência no rendimento e na pureza do produto. Desta forma, o presente estudo propõe a reutilização do CP, no processo de extração de P(3HB), contribuindo assim para a redução dos custos de produção deste biopolímero.
Palavras-chave
Abstract
Poly(3-hydroxybutyrate) (P(3HB)) extraction using toxic volatile solvents is contradictory to the idea of using this biopolymer to minimize environmental aggressions caused by petrochemical polymers. Propylene carbonate (PC) has low toxicity and high boiling point, which ensure a low risk of losses due to evaporation and high recovery performance, allowing for reuse. The aim of this study was to investigate the effect of reusing PC recovered the process of extraction of P(3HB) produced by Cupriavidus necator on the performance and purity of the biopolymer and evaluate the amount of solvent loss during the stages of the extraction process. It was observed that 80% of PC could be recovered throughout the process. The recovered solvent was reused eleven times without losing its efficiency in terms of product performance and purity. Hence, the present study proposes to reuse PC in the process of P(3HB) extraction, thus contributing to reduce the costs for the production of this biopolymer.
Keywords
References
1. Anderson, A. J., & Dawes, E. A. (1990). Occurrence, metabolism, metabolic role and industrial uses of bacterial polyhydroxyalkanoates. Microbiology Reviews, 54(4), 450-472. http://dx.doi.org/0146-0749/90/040450-23.
2. Khanna, S., & Srivastava, A. K. (2004). Recent advances in microbial polyhydroxyalkanoates. Process Biochemistry, 40(2), 607-619. http://dx.doi.org/10.1016/j.procbio.2004.01.053.
3. Madkour, M. H., Heinrich, D., Alghamdi, M. A., Shabbaj, I. I., & Steinbüchel, A. (2013). PHA recovery from biomass. Biomacromolecules, 14(9), 2963-2972. PMid:23875914. http://dx.doi.org/10.1021/bm4010244.
4. Silva, L. F., Gomez, J. G. C., Rocha, R. C. S., Taciro, M. K., & Pradella, J. G. C. (2007). Produção biotecnológica de poli-hidroxialcanoatos para a geração de polímeros biodegradáveis no Brasil. Quimica Nova, 30(7), 1732-1743. http://dx.doi.org/10.1590/S0100-40422007000700040.
5. Kshirsagar, P. R., Kulkarni, S. O., Nilegaonkar, S. S., Niveditha, M., & Kanekar, P. P. (2013). Kinetics and model building for recovery of polyhydroxyalkanoate (PHA) from Halomonas campisalis. Separation and Purification Technology, 130, 151-160. http://dx.doi.org/10.1016/j.seppur.2012.10.007.
6. Fiorese, M. L., Freitas, F., Pais, J., Ramos, A. M., Aragão, G. M. F., & Reis, M. A. M. (2009). Recovery of polyhydroxybutyrate (PHB) from Cupriavidus necator biomass by solvent extraction with 1,2-propylene carbonate. Engineering in Life Sciences, 9(6), 454-461.http://dx.doi.org/10.1002/elsc.200900034.
7. Quines, L. K. M., Ienczak, J. L., Schmidt, M., Zanfonato, K., Rodrigues, M. I., Schmidell, W., & Aragão, G. M. F. (2015). Extração de poli(3-hidroxibutirato), produzido por Cupriavidus necator, com carbonato de propileno. Quimica Nova, 38(2), 214-220. http://dx.doi.org/10.5935/0100-4042.20140297.
8. Ramsay, J. A., Berger, E., Voyer, R., Chavarie, C., & Ramsay, B. A. (1994). Extraction of poli-3-hydroxybutyrate using chlorinated solvents. Biotechnology Techniques, 8(8), 589-594. http://dx.doi.org/10.1007/BF00152152.
9. Neves, A., & Müller, J. (2012). Use of enzymes in extraction of polyhydroxyalkanoates produced by Cupriavidus necator. Biotechnology Progress, 28(6), 1575-1580. PMid:22915526. http://dx.doi.org/10.1002/btpr.1624.
10. Martino, L. L., Cruz, M. V., Scomac, A., Freitas, F., Bertin, L., Scandola, M., & Reis, M. A. M. (2014). Recovery of amorphous polyhydroxybutyrate granules from Cupriavidus necator cells grown on used cooking oil. Journal of Biological Macromolecules, 71, 117-123. PMid:24751509.http://dx.doi.org/10.1016/j.ijbiomac.2014.04.016.
11. Tamer, I. M., Moo-Young, M., & Chist, Y. (1998). Optimization of poly(beta-hydroxybutyric acid) recovery from Alcaligenes latus: combined mechanical and chemical treatments. Bioprocess Engineering, 19(6), 459-468. http://dx.doi.org/10. 1007/PL00009030.
12. Van Hee, P., Middelberg, A. P. J., Van Der, L. R. G. J. M., & Van Der, W. L. A. M. (2004). Relation between cell disruption conditions, cell debris particle size, and inclusion body release. Biotechnology and Bioengineering, 88(1), 100-110. PMid:15449302. http://dx.doi.org/10.1002/bit.20343.
13. Chen, Y., Yang, H., Zhou, Q., Chen, J., & Gu, G. (2001). Cleaner recovery of poly(3-hydroxy-butyric acid) synthesized in Alcaligenes eutrophus. Process Biochemistry, 36(6), 501-506. http://dx.doi.org/10.1016/S0032-9592(00)00225-9.
14. Jiang, Y., Mikova, G., Kleerebezem, R., Wielen, L. A. M., & Cuellar, M. C. (2015). Feasibility study of an alkaline based chemical treatment for the purification of polyhydroxybutyrate produced by a mixed enriched culture. AMB Express, 5(1), 1-13. PMid:25642402. http://dx.doi.org/10.1186/s13568-015-0096-5.
15. Riedel, S. L., Brigham, C. J., Budde, C. F., Bader, J., Rha, C., Stahl, U., & Sinskey, A. J. (2013). Recovery of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from Ralstonia eutrophacultures with non-halogenated solvents. Biotechnology and Bioengineering, 110(2), 461-470. PMid:22903730. http://dx.doi.org/10.1002/bit.24713.
16. Mcchalicher, C. W. J., Srienc, F., & Rouse, D. P. (2009). Solubility and degradation of polyhydroxyalkanoate biopolymers in propylene carbonate. American Institute of Chemical Engineers, 56(6), 1616-1625. http://dx.doi.org/10.1002/aic.12087.
18. Cosmetic Ingredient Review – CIR. (1987). Final report on the assessment of propylene carbonate. Journal of the American College of Toxicology, 6(1), 23-51. http://dx.doi.org/10.3109/10915818709095488.
19. Environmental Protection Agency. (1998). Environmental profile for propylene carbonate (pp. 1-52). Cincinnati: United States Environmental Protection Agency. Recuperado em 19 de março de 2015, de http://nepis.epa.gov
20. Karr, D. B., Waters, J. K., & Emerich, D. W. (1983). Analysis of Poly-3-Hydroxybutyrate in Rhizobium japonicum Bacteroids by ion-exclusion high-pressure liquid chromatography and UV detectiont. Applied and Environmental Microbiology, 46(6), 1339-1344. PMid:16346443.
21. Aragão, G. M. F., Lindley, N. D., Uribelarrea, J. L., & Pareilleux, A. (1996). Maintaining a controlled residual growth capacity increases the production of polyhydroxyalkanoate copolymers by Alcaligenes eutrophus. Biotechnology Letters, 18(8), 937-942. http://dx.doi.org/10.1007/BF00154625.
22. Canevarolo, S. V. (2007). Técnicas de caracterização de polímeros. São Paulo: ARTLIBER. 444 p.
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