Polímeros: Ciência e Tecnologia
https://revistapolimeros.org.br/article/doi/10.1590/0104-1428.20240029
Polímeros: Ciência e Tecnologia
Original Article

Development of nano-antimicrobial material based on bacterial cellulose, silver nanoparticles, and ClavaninA

Glícia Maria Oliveira; Alberto Galdino Silva-Junior; Octávio Luiz Franco; José Lamartine de Andrade Aguiar; Flávia Cristina Morone Pinto; Reginaldo Gonçalves de Lima-Neto; Maria Danielly Lima de Oliveira; César Augusto Souza de Andrade

http://dx.doi.org/10.1590/0104-1428.20240029 Polímeros: Ciência e Tecnologia, vol.34, n3, e20240030, 2024
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Abstract

This study presents a novel approach to obtaining nano-antimicrobial hybrid material by integrating electrospun nanofibers based on cellulosic biopolymer (BP) associated with antimicrobial agents, specifically silver nanoparticles (AgNPs) and Clavanin A (ClavA), an antimicrobial peptide obtained from the marine tunicate Styela clava. The electrospinning technique produced the blended polyvinyl alcohol:BP nanofibers. Chemical crosslinking was performed to ensure the stability of the nanofibers. The nanofibers had an average diameter of 568 nm for PVA nanofibers and 648 nm for PVA nanofibers functionalized with silver nanoparticles. The nanohybrid material demonstrates significant inhibition zones against Gram-positive (Bacillus subtilis, Staphylococcus aureus) and Gram-negative (Pseudomonas aeruginosa, Klebsiella pneumoniae) bacteria. P. aeruginosa exhibits a substantial inhibition zone of 15 mm. Thus, the nanohybrid material was effective against this challenging pathogen. Combining electrospun nanofibers, bacterial cellulose hydrogel, and antimicrobial agents establishes a solution that could combat microbial threats in wound care.

 

 

Keywords

antimicrobial peptide, Clavanin A, electrospinning, silver nanoparticles, sugarcane biopolymer

References

1 Caldwell, M. D. (2020). Bacteria and antibiotics in wound healing. The Surgical Clinics of North America, 100(4), 757-776. http://doi.org/10.1016/j.suc.2020.05.007. PMid:32681875.

2 Sen, C. K., Gordillo, G. M., Roy, S., Kirsner, R., Lambert, L., Hunt, T. K., Gottrup, F., Gurtner, G. C., & Longaker, M. T. (2009). Human skin wounds: a major and snowballing threat to public health and the economy. Wound Repair and Regeneration, 17(6), 763-771. http://doi.org/10.1111/j.1524-475X.2009.00543.x. PMid:19903300.

3 Hurlow, J., & Bowler, P. G. (2022). Acute and chronic wound infections: microbiological, immunological, clinical and therapeutic distinctions. Journal of Wound Care, 31(5), 436-445. http://doi.org/10.12968/jowc.2022.31.5.436. PMid:35579319.

4 Shalaby, M. A., Anwar, M. M., & Saeed, H. (2022). Nanomaterials for application in wound healing: current state-of-the-art and future perspectives. Journal of Polymer Research, 29(3), 91. http://doi.org/10.1007/s10965-021-02870-x.

5 Kolimi, P., Narala, S., Nyavanandi, D., Youssef, A. A. A., & Dudhipala, N. (2022). (Year). Innovative treatment strategies to accelerate wound healing: trajectory and recent advancements. Cells, 11(15), 2439. http://doi.org/10.3390/cells11152439. PMid:35954282.

6 Singh, B. K., & Dutta, P. K. (2015). Chitin, chitosan, and silk fibroin electrospun nanofibrous scaffolds: A prospective approach for regenerative medicine. In S. Kalia (Ed.), Chitin and chitosan for regenerative medicine (pp. 151-189). New Delhi: Springer. http://doi.org/10.1007/978-81-322-2511-9_7.

7 Zhang, W., Ronca, S., & Mele, E. (2017). Electrospun nanofibres containing antimicrobial plant extracts. Nanomaterials, 7(2), 42. http://doi.org/10.3390/nano7020042. PMid:28336874.

8 Gao, C., Zhang, L., Wang, J., Jin, M., Tang, Q., Chen, Z., Cheng, Y., Yang, R., & Zhao, G. (2021). Electrospun nanofibers promote wound healing: theories, techniques, and perspectives. Journal of Materials Chemistry. B, Materials for Biology and Medicine, 9(14), 3106-3130. http://doi.org/10.1039/D1TB00067E. PMid:33885618.

9 Nadaf, A., Gupta, A., Hasan, N., Fauziya, Ahmad, S., Kesharwani, P., & Ahmad, F. J. (2022). Recent update on electrospinning and electrospun nanofibers: current trends and their applications. RSC Advances, 12(37), 23808-23828. http://doi.org/10.1039/D2RA02864F. PMid:36093244.

10 Oliveira, G. M., Gomes, A. O., Fo., Silva, J. G. M., Silva, A. G., Jr., Lins, E. M., Oliveira, M. D. L., & Andrade, C. A. S. (2023). Bacterial cellulose biomaterials for the treatment of lower limb ulcers. Revista do Colégio Brasileiro de Cirurgiões, 50(1), e20233536. http://doi.org/10.1590/0100-6991e-20233536-en. PMid:37222383.

11 Silva, J. G. M., Pinto, F. C. M., Oliveira, G. M., Silva, A. A., Campos, O., Jr., Silva, R. O., Teixeira, V. W., Melo, I. M. F., Paumgartten, F. J. R., Souza, T. P., Carvalho, R. R., Oliveira, A. C. A. X., Aguiar, J. L. A., & Teixeira, Á. A. C. (2020). Non-clinical safety study of a sugarcane bacterial cellulose hydrogel. Research, Society and Development, 9(9), e960997932. http://doi.org/10.33448/rsd-v9i9.7932.

12 Pinto, F. C. M., De-Oliveira, A. C. A. X., De-Carvalho, R. R., Gomes-Carneiro, M. R., Coelho, D. R., Lima, S. V. C., Paumgartten, F. J. R., & Aguiar, J. L. A. (2016). Acute toxicity, cytotoxicity, genotoxicity and antigenotoxic effects of a cellulosic exopolysaccharide obtained from sugarcane molasses. Carbohydrate Polymers, 137, 556-560. http://doi.org/10.1016/j.carbpol.2015.10.071. PMid:26686163.

13 Gurunathan, S. (2019). Rapid biological synthesis of silver nanoparticles and their enhanced antibacterial effects against Escherichia fergusonii and Streptococcus mutans. Arabian Journal of Chemistry, 12(2), 168-180. http://doi.org/10.1016/j.arabjc.2014.11.014.

14 Behravan, M., Panahi, A. H., Naghizadeh, A., Ziaee, M., Mahdavi, R., & Mirzapour, A. (2019). Facile green synthesis of silver nanoparticles using Berberis vulgaris leaf and root aqueous extract and its antibacterial activity. International Journal of Biological Macromolecules, 124, 148-154. http://doi.org/10.1016/j.ijbiomac.2018.11.101. PMid:30447360.

15 Kalaivani, R., Maruthupandy, M., Muneeswaran, T., Hameedha Beevi, A., Anand, M., Ramakritinan, C. M., & Kumaraguru, A. K. (2018). Synthesis of chitosan mediated silver nanoparticles (Ag NPs) for potential antimicrobial applications. Frontiers in Laboratory Medicine, 2(1), 30-35. http://doi.org/10.1016/j.flm.2018.04.002.

16 Singh, H., Du, J., Singh, P., & Yi, T. H. (2018). Extracellular synthesis of silver nanoparticles by Pseudomonas sp. THG-LS1.4 and their antimicrobial application. Journal of Pharmaceutical Analysis, 8(4), 258-264. http://doi.org/10.1016/j.jpha.2018.04.004. PMid:30140490.

17 Guzmán, M. G., Dille, J., & Godet, S. (2009). Synthesis of silver nanoparticles by chemical reduction method and their antibacterial activity. International Journal of Chemical and Biomolecular Engineering, 2(3), 104-111.

18 Pasupuleti, M., Schmidtchen, A., & Malmsten, M. (2012). Antimicrobial peptides: key components of the innate immune system. Critical Reviews in Biotechnology, 32(2), 143-171. http://doi.org/10.3109/07388551.2011.594423. PMid:22074402.

19 Xu, J., Li, Y., Wang, H., Zhu, M., Feng, W., & Liang, G. (2021). Enhanced antibacterial and anti-biofilm activities of antimicrobial peptides modified silver nanoparticles. International Journal of Nanomedicine, 16, 4831-4846. http://doi.org/10.2147/IJN.S315839. PMid:34295158.

20 Browne, K., Chakraborty, S., Chen, R., Willcox, M. D., Black, D. S., Walsh, W. R., & Kumar, N. (2020). A new era of antibiotics: the clinical potential of antimicrobial peptides. International Journal of Molecular Sciences, 21(19), 7047. http://doi.org/10.3390/ijms21197047. PMid:32987946.

21 Santajit, S., & Indrawattana, N. (2016). Mechanisms of antimicrobial resistance in ESKAPE pathogens. BioMed Research International, 2016, 2475067. http://doi.org/10.1155/2016/2475067. PMid:27274985.

22 Kalan, L. R., & Brennan, M. B. (2019). The role of the microbiome in nonhealing diabetic wounds. Annals of the New York Academy of Sciences, 1435(1), 79-92. http://doi.org/10.1111/nyas.13926. PMid:30003536.

23 Paterson-Beedle, M., Kennedy, J. F., Melo, F. A. D., Lloyd, L. L., & Medeiros, V. (2000). A cellulosic exopolysaccharide produced from sugarcane molasses by a Zoogloea sp. Carbohydrate Polymers, 42(4), 375-383. http://doi.org/10.1016/S0144-8617(99)00179-4.

24 Ullah, S., Hashmi, M., Hussain, N., Ullah, A., Sarwar, M. N., Saito, Y., Kim, S. H., & Kim, I. S. (2020). Stabilized nanofibers of polyvinyl alcohol (PVA) crosslinked by unique method for efficient removal of heavy metal ions. Journal of Water Process Engineering, 33, 101111. http://doi.org/10.1016/j.jwpe.2019.101111.

25 Acharya, D., Mohanta, B., Pandey, P., Singha, M., & Nasiri, F. (2017). Optical and antibacterial properties of synthesised silver nanoparticles. Micro & Nano Letters, 12(4), 223-226. http://doi.org/10.1049/mnl.2016.0666.

26 İspir, E., Toroğlu, S., & Kayraldız, A. (2008). Syntheses, characterization, antimicrobial and genotoxic activities of new Schiff bases and their complexes. Transition Metal Chemistry, 33(8), 953-960. http://doi.org/10.1007/s11243-008-9135-2.

27 Abdul Khalil, H. P. S., Davoudpour, Y., Islam, M. N., Mustapha, A., Sudesh, K., Dungani, R., & Jawaid, M. (2014). Production and modification of nanofibrillated cellulose using various mechanical processes: a review. Carbohydrate Polymers, 99, 649-665. http://doi.org/10.1016/j.carbpol.2013.08.069. PMid:24274556.

28 Qashou, S. I., El-Zaidia, E. F. M., Darwish, A. A. A., & Hanafy, T. A. (2019). Methylsilicon phthalocyanine hydroxide doped PVA films for optoelectronic applications: FTIR spectroscopy, electrical conductivity, linear and nonlinear optical studies. Physica B, Condensed Matter, 571, 93-100. http://doi.org/10.1016/j.physb.2019.06.063.

29 Bai, J., Li, Y., Yang, S., Du, J., Wang, S., Zheng, J., Wang, Y., Yang, Q., Chen, X., & Jing, X. (2007). A simple and effective route for the preparation of poly(vinylalcohol) (PVA) nanofibers containing gold nanoparticles by electrospinning method. Solid State Communications, 141(5), 292-295. http://doi.org/10.1016/j.ssc.2006.10.024.

30 Sofla, M. R. K., Brown, R. J., Tsuzuki, T., & Rainey, T. J. (2016). A comparison of cellulose nanocrystals and cellulose nanofibres extracted from bagasse using acid and ball milling methods. Advances in Natural Sciences: Nanoscience and Nanotechnology, 7(3), 035004. http://doi.org/10.1088/2043-6262/7/3/035004.

31 Ghozali, M., Meliana, Y., & Chalid, M. (2021). Synthesis and characterization of bacterial cellulose by Acetobacter xylinum using liquid tapioca waste. Materials Today: Proceedings, 44(Part 1), 2131-2134. http://doi.org/10.1016/j.matpr.2020.12.274.

32 Hameed, M. M. A., Khan, S. A. P. M., Thamer, B. M., Al-Enizi, A., Aldalbahi, A., El-Hamshary, H., & El-Newehy, M. H. (2021). Core-shell nanofibers from poly(vinyl alcohol) based biopolymers using emulsion electrospinning as drug delivery system for cephalexin drug. Journal of Macromolecular Science, Part A: Pure and Applied Chemistry, 58(2), 130-144. http://doi.org/10.1080/10601325.2020.1832517.

33 Nasikhudin, D., Diantoro, M., Kusumaatmaja, A., & Triyana, K. (2016). Preparation of PVA/Chitosan/TiO2 nanofibers using electrospinning method. AIP Conference Proceedings, 1755(1), 150002. http://doi.org/10.1063/1.4958575.

34 Gopiraman, M., Deng, D., Saravanamoorthy, S., Chung, I.-M., & Kim, I. S. (2018). Gold, silver and nickel nanoparticle anchored cellulose nanofiber composites as highly active catalysts for the rapid and selective reduction of nitrophenols in water. RSC Advances, 8(6), 3014-3023. http://doi.org/10.1039/C7RA10489H. PMid:35541203.

35 Blanes, M., Gisbert, M. J., Marco, B., Bonet, M., Gisbert, J., & Balart, R. (2010). Influence of glyoxal in the physical characterization of PVA nanofibers. Textile Research Journal, 80(14), 1465-1472. http://doi.org/10.1177/0040517509357654.

36 Oliveira, A. H. P., Moura, J. A. S., & Oliveira, H. P. (2013). Preparação e caracterização de microfibras de poli(álcool vinílico)/dióxido de titânio. Polímeros: Ciência e Tecnologia, 23(2), 196-200. http://doi.org/10.1590/S0104-14282013005000013.

37 van Etten, E. A., Ximenes, E. S., Tarasconi, L. T., Garcia, I. T. S., Forte, M. M. C., & Boudinov, H. (2014). Insulating characteristics of polyvinyl alcohol for integrated electronics. Thin Solid Films, 568, 111-116. http://doi.org/10.1016/j.tsf.2014.07.051.

38 Abeykoon, S. W., & White, R. J. (2022). Continuous square wave voltammetry for high information content interrogation of conformation switching sensors. ACS Measurement Science Au, 3(1), 1-9. http://doi.org/10.1021/acsmeasuresciau.2c00044. PMid:36817008.

39 Katouah, H. A., El-Sayed, R., & El-Metwaly, N. M. (2021). Solution blowing spinning technology and plasma-assisted oxidation-reduction process toward green development of electrically conductive cellulose nanofibers. Environmental Science and Pollution Research International, 28(40), 56363-56375. http://doi.org/10.1007/s11356-021-14615-w. PMid:34050912.

40 Khamwongsa, P., Wongjom, P., Cheng, H., Lin, C. C., & Ummartyotin, S. (2022). Significant enhancement of electrical conductivity of conductive cellulose derived from bamboo and polypyrrole. Composites Part C: Open Access, 9, 100314. http://doi.org/10.1016/j.jcomc.2022.100314.

41 Meirinho, S. G., Dias, L. G., Peres, A. M., & Rodrigues, L. R. (2017). Electrochemical aptasensor for human osteopontin detection using a DNA aptamer selected by SELEX. Analytica Chimica Acta, 987, 25-37. http://doi.org/10.1016/j.aca.2017.07.071. PMid:28916037.

42 Miranda, J. L., Oliveira, M. D. L., Oliveira, I. S., Frias, I. A. M., Franco, O. L., & Andrade, C. A. S. (2017). A simple nanostructured biosensor based on clavanin A antimicrobial peptide for gram-negative bacteria detection. Biochemical Engineering Journal, 124, 108-114. http://doi.org/10.1016/j.bej.2017.04.013.

43 Silva, O. N., Fensterseifer, I. C. M., Rodrigues, E. A., Holanda, H. H. S., Novaes, N. R. F., Cunha, J. P. A., Rezende, T. M. B., Magalhães, K. G., Moreno, S. E., Jerônimo, M. S., Bocca, A. L., & Franco, O. L. (2015). Clavanin A improves outcome of complications from different bacterial infections. Antimicrobial Agents and Chemotherapy, 59(3), 1620-1626. http://doi.org/10.1128/AAC.03732-14. PMid:25547358.

44 Villarreal-Gómez, L. J., Pérez-González, G. L., Bogdanchikova, N., Pestryakov, A., Nimaev, V., Soloveva, A., Cornejo-Bravo, J. M., & Toledaño-Magaña, Y. (2021). Antimicrobial effect of electrospun nanofibers loaded with silver nanoparticles: influence of Ag incorporation method. Journal of Nanomaterials, 2021, e9920755. http://doi.org/10.1155/2021/9920755.

45 Gromovykh, T. I., Vasil’kov, A. Yu., Sadykova, V. S., Feldman, N. B., Demchenko, A. G., Lyundup, A. V., Butenko, I. E., & Lutsenko, S. V. (2019). Creation of composites of bacterial cellulose and silver nanoparticles: evaluation of antimicrobial activity and cytotoxicity. International Journal of Nanotechnology, 16(6-10), 408-420. http://doi.org/10.1504/IJNT.2019.106615.

46 Garza-Cervantes, J. A., Mendiola-Garza, G., Macedo de Melo, E., Dugmore, T. I. J., Matharu, A. S., & Morones-Ramirez, J. R. (2020). Antimicrobial activity of a silver-microfibrillated cellulose biocomposite against susceptible and resistant bacteria. Scientific Reports, 10(1), 7281. http://doi.org/10.1038/s41598-020-64127-9. PMid:32350328.
 

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