Polímeros: Ciência e Tecnologia
https://revistapolimeros.org.br/article/doi/10.1590/0104-1428.20240029
Polímeros: Ciência e Tecnologia
Original Article

Development of nano-antimicrobial material based on bacterial cellulose, silver nanoparticles, and ClavaninA

Glícia Maria Oliveira; Alberto Galdino Silva-Junior; Octávio Luiz Franco; José Lamartine de Andrade Aguiar; Flávia Cristina Morone Pinto; Reginaldo Gonçalves de Lima-Neto; Maria Danielly Lima de Oliveira; César Augusto Souza de Andrade

http://dx.doi.org/10.1590/0104-1428.20240029 Polímeros: Ciência e Tecnologia, vol.34, n3, e20240030, 2024
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Abstract

This study presents a novel approach to obtaining nano-antimicrobial hybrid material by integrating electrospun nanofibers based on cellulosic biopolymer (BP) associated with antimicrobial agents, specifically silver nanoparticles (AgNPs) and Clavanin A (ClavA), an antimicrobial peptide obtained from the marine tunicate Styela clava. The electrospinning technique produced the blended polyvinyl alcohol:BP nanofibers. Chemical crosslinking was performed to ensure the stability of the nanofibers. The nanofibers had an average diameter of 568 nm for PVA nanofibers and 648 nm for PVA nanofibers functionalized with silver nanoparticles. The nanohybrid material demonstrates significant inhibition zones against Gram-positive (Bacillus subtilis, Staphylococcus aureus) and Gram-negative (Pseudomonas aeruginosa, Klebsiella pneumoniae) bacteria. P. aeruginosa exhibits a substantial inhibition zone of 15 mm. Thus, the nanohybrid material was effective against this challenging pathogen. Combining electrospun nanofibers, bacterial cellulose hydrogel, and antimicrobial agents establishes a solution that could combat microbial threats in wound care.

 

 

Keywords

antimicrobial peptide, Clavanin A, electrospinning, silver nanoparticles, sugarcane biopolymer

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