Polímeros: Ciência e Tecnologia
https://revistapolimeros.org.br/article/doi/10.1590/S0104-14282005000200017
Polímeros: Ciência e Tecnologia
Scientific & Technical Article

Blends of Agar/Galactomannan for Marubakaido Apple Rootstock Shoot Proliferation

Sierakowski, Maria Rita; Anjos, A.; Quoirin, M.; Lucyszyn, N.

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Abstract

Galactomannans (GMs) extracted from seeds of a native Brazilian specie designated Cassia fastuosa (cassia) and from Cyamopsis tetragonolobus (guar gum – a commercial GM) were mixed with agar, in the proportion of 3/3 g.L-1 (w/w), and used as a gelling agent in Marubakaido apple rootstock (Malus prunifolia Borkh) micropropagation. The rheological measurements of the gels showed a good interaction between both hidrocolloids. The micropropagation results on these media were compared with behaviour of standard medium containing agar only (6 g.L-1). Results from in vitro experiments indicated enhanced proliferation of apple shoots and also the occurrence of hyperhydric shoots was lower in the modified gel-medium.

Keywords

Galactomannan, micropropagation, apple tree, gelled medium

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