Polímeros: Ciência e Tecnologia
Polímeros: Ciência e Tecnologia
Original Article

Polysaccharides of red alga Gracilaria intermedia: structure, antioxidant activity and rheological behavior

Joana Paula Lima de Castro; Luís Eduardo Castanheira Costa; Maísa Pessoa Pinheiro; Thiago dos Santos Francisco; Pedro Hermano Menezes de Vasconcelos; Lizandra Mistrello Funari; Renata Moschini Daudt; Gustavo Ramalho Cardoso dos Santos; Nilo Sérgio Medeiros Cardozo; Ana Lúcia Ponte Freitas

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Abstract: A sulfated polysaccharide fraction from the red alga Gracilaria intermedia (PLS) was obtained by papain digestion (60 °C, 30 min). The extract was subjected to colorimetry and turbidimetry analysis, Fourier transform infrared (FTIR) spectroscopy, 1H, 13 C and 2D 1H COSY nuclear magnetic resonance (NMR) and gas chromatography/mass spectrometry analysis. Antioxidant activity tests were performed (chelation of ferrous ion, total antioxidant capacity, and scavenging of DPPH radicals); significant activity of the extract indicated that these polysaccharides may be used as non-synthetic antioxidants. The rheological behavior of aqueous polysaccharide solutions was studied at 25 ± 1 °C using steady-shear and dynamic oscillatory measurements. All the solutions analyzed showed pseudoplastic behavior and potential to act as a thickening agent, as proved through a preliminary comparison with a commercial product used for this application.


antioxidant activities, Gracilaria intermedia, polysaccharides, rheological behavior, structure


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